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The gonads were pre-hybridized in PBST/hybridization buffer (1:1) for 5 mins at 48C, followed by 1 hour of incubation in hybridization buffer

The observed synergy is not limited to GPxs and Prxs and other agents, which are antioxidants could reduce H 2 O 2 at the cellular level and also have a synergetic coupling with the GPxs and Prxs

When bound to a cis-regulatory element, known as an antioxidant response, the element in the promoter region of antioxidant enzyme genes boosts the transcriptional expression (Hopkins, 2017)

When injected into targeted areas, it temporarily paralyses the nerves, preventing them from signalling the sweat glands to produce excessive sweat