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GST-OLFM4 protein was expressed in BL21 (DE3) by 0.5 M IPTG inducing overnight, then harvest cells and resuspend in phosphate-buffered saline (PBS) (0.1% Triton100, Protease Inhibitor Cocktail, 1 mM PMSF), after lyzed by sonication, proteins were purified by glutathione Sepharose 4B (GE healthcare)

Transcriptome profiling indicated that the average relative expression of 16 DEGs were higher than 10, the relative expression of the 5 DEGs were higher than 100

A single measurement without clinical context may not reflect true intracellular glutathione availability or functional capacity

A man can have a clinically normal total testosterone reading while carrying substantially lower free testosterone because of SHBG genetics