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At varying time points (0, 2, 5, 15, 30 min), purified Pt GSTF8 (8 M) and GSH (20 M) were added to an aliquot of the equilibration reaction and the resulting mixtures were incubated at 30 C for 30 min at 1200 rpm using a 3 mm shaking diameter before extraction with 2 vol of 75% methanol in H 2 O, 1 mM EDTA, 0.3% hydrochloric acid

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In addition, the proposed structureactivity relationships are based on a limited set of compounds, making it difficult to establish definitive correlations regarding the influence of arene ligands, curcuminoid backbones, or PTA coordination on biological behaviour

When combined with slow COMT and low SULT1A1, this leaves only non-enzymatic antioxidant defenses against reactive 4-OHE2 quinones